Detection of soybean allergens based on isotope dilution mass
Therefore, the isotope dilution mass spectrometry (IDMS) is developed as a calibration method to eliminate the matrix effects with the addition of the known amount of isotopically labeled analogues served as internal standards [32], [33], [34].
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Isotope-Dilution Mass Spectrometry in Clinical Chemistry
This chapter discusses isotope dilution mass spectrometry in clinical chemistry. Mass spectrometry (MS) in clinical chemistry is increasingly used as an analytical tool to solve complex problems in biochemistry and medicine. As a …
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Validation of an isotope dilution mass spectrometry (IDMS …
An isotope dilution two-step solid-phase extraction followed by an ultra-performance liquid chromatography separation coupled to tandem mass spectrometry (UPLC-MS/MS) detection method was validated for the quantification of 21 steroid hormones (androgens, estrogens, glucocorticoids, and progestogens) in whole waters.
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Introduction to Isotope Dilution Mass Spectrometry (IDMS)
By a combination of sample pre-concentration and Isotope Dilution Mass Spectrometry (IDMS), it was possible to determine isotopic ratio in soil extracts with a glycine concentration of only 3 µM ...
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Isotope dilution mass spectrometry — A primary method of …
This article describes the application of isotope dilution mass spectrometry (IDMS) to the field of reference material (RM) characterisation focusing …
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Isotope Dilution Mass Spectrometry For Quantitative …
Isotope Dilution Mass Spectrometry For Quantitative Elemental Analysis Of Powdered Samples By Radiofrequency Pulsed Glow Discharge Time Of Flight Mass Spectrometry. Published. October 15, 2013. Author(s) John L. Molloy, Julian Malherbe, Fanny Claverie, Beatriz Fernandez, Aitor Alvarez-Toral, Alfredo Sanz-Medel, Rosario …
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Quantitation of glycated albumin by isotope dilution mass spectrometry
The aim of our study was to develop an assay for absolute quantitation of glycated albumin based on isotope dilution liquid chromatography-mass spectrometry. Methods: The plasma samples were reduced/alkylated, spiked with isotope-labeled standards RQIKKQTALV(D 8 )E and RQIKK(fructosyl)QTALV(D 8 )E and enzymatically digested …
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Isotope dilution | Mass spectrometry, Trace elements, …
Isotope dilution, radiochemical method of analysis for measuring the mass and quantity of an element in a substance. The procedure involves adding to a substance a known quantity of a radioisotope of the element to be measured and mixing it with the stable isotope of the element. A sample is then
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Isotope Dilution Mass Spectrometry
when mass spectrometry more and more evolved into an analytical technique of highest performance, this principle has been combined with mass spectrometry, which now is known as Isotope Dilution Mass Spectrometry (IDMS). An IDMS precursor has been published in biochemistry. Here the metabolism of amino acids in animals has been …
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Isotope Dilution Mass Spectroscopy
Isotope dilution mass spectrometry (IDMS) is an analytical technique based on the modification of the natural isotope composition of elements or compounds after the addition to the sample of an enriched isotope or an isotopically labeled form of the analyte.
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Evaluation of different isotope dilution mass spectrometry …
Natural abundance and isotopically labelled tryptic peptides are routinely employed as standards in quantitative proteomics. The certification of the peptide content is usually carried out by amino acid analysis using isotope dilution mass spectrometry (IDMS) after the acid hydrolysis of the peptide. For the validation and traceability of the …
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Isotope-dilution liquid chromatography-tandem mass spectrometry …
Isotope dilution mass spectrometry (ID-MS) method is typically performed with trypsin digestion followed by analysis of signature peptides produced. Stable isotope-labeled (SIL) peptides or protein analogs are used as internal standards (ISs) to improve the accuracy of the assay by normalizing matrix effects. LC-MS/MS method can …
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Guidelines for Achieving High Accuracy in Isotope Dilution …
The isotope dilution mass spectrometry (IDMS) technique is well known and widely reported in the literature. However, its application can present considerable …
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Development of an isotope dilution mass spectrometry …
An isotope dilution mass spectrometry (IDMS) method that involves peptide-based protein analysis was developed to accurately quantify insulin. In this study, a signature peptide (GFFYTPK) obtained from tryptic digestion of insulin was selected as a surrogate for insulin. Then, the optimal conditions for signature peptide analysis through …
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Calibration graphs in isotope dilution mass spectrometry
Theoretical considerations in stable isotope dilution mass spectrometry for organic analysis. Anal. Chem., 48 (1976), pp. 1885-1890, 10.1021/ac50007a019. View in Scopus Google Scholar [14] B.H. Min, W.A. Garland. Determination of clonazepam and its 7-amino metabolite in plasma and blood by gas chromatography—chemical ionization …
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Isotope-dilution mass spectrometry for exact …
Isotope-dilution mass spectrometry for exact quantification of noncanonical DNA nucleosides Franziska R. Traube 1,2, Sarah Schiffers 1,2, Katharina Iwan 1,2, Stefanie Kellner 1, Fabio Spada 1,
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An Accurate Isotope Dilution Liquid Chromatography-Tandem Mass
Isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC–MS/MS) is widely accepted as a reference method for numerous clinical indicators, including C-peptide, owing to its specificity, accuracy, and sensitivity [10, 11]. To improve the harmonization of serum C-peptide measurements and assign the values of control …
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Isotope Dilution Mass Spectrometry
Isotope Dilution Mass Spectrometry (IDMS) has become an essential tool in research laboratories and is increasingly used in routine analysis labs (including environmental, food safety and clinical applications). This is the first textbook to present a comprehensive and instructive view of the theory and applications of this growing technique.
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Isotope-dilution mass spectrometry for exact quantification …
Isotopically labeled standards are added in the course of DNA digestion, which allows exact quantification by isotope dilution mass spectrometry. To …
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Method 6800: Elemental and Molecular Speciated …
1.1 This method consists of two approaches: (1) isotope dilution mass spectrometry (IDMS) for the determination of the total concentrations of elements and molecules and (2) molecular speciated isotope dilution mass spectrometry (SIDMS) for the determination of elemental and molecular species.
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Guidelines for Achieving High Accuracy in Isotope Dilution Mass
The isotope dilution mass spectrometry (IDMS) technique is well known and widely reported in the literature. However, its application can present considerable difficulties with regard to obtaining reliable results. Produced jointly by the Royal Society of Chemistry's Analytical Methods Committee and the Valid Analytical Measurement (VAM ...
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Towards absolute quantification of therapeutic monoclonal …
The method combines liquid chromatography tandem mass spectrometry (LC-MS/MS) and protein cleavage isotope dilution mass spectrometry with the isotope-labeled mAb as IS. The latter was identical to the analyzed mAb with the exception that each threonine contains four (13)C atoms and one (15)N atom. Serum samples were …
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Determination of Cystatin C in human serum by isotope dilution mass
We propose a peptide-based isotope dilution mass spectrometry approach for Cystatin C determination in human serum samples, a clinical marker for renal status for which backup by a mass spectrometry based primary method has been missing so far. In contrast to common protocols, the isotope labelled version of the proteotypic signature …
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Isotope dilution mass spectrometry as an independent …
1. Introduction. Isotope dilution mass spectrometry (IDMS) is an established method for measuring amount of an element and, when carefully implemented, can be a primary measurement method that is traceable to the mole [[1], [2], [3]].The measurements described here represent a novel use of IDMS to precisely quantify the …
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(PDF) Isotope Dilution Mass Spectrometry
This article describes the application of isotope dilution mass spectrometry (IDMS) to the field of reference material (RM) characterisation focusing on the approach, which is applied by the IDMS ...
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On-line isotope dilution analysis with the 7700 Series …
Isotope dilution analysis (IDA) or isotope dilution mass spectrometry (ID-MS) is a well-known analytical technique based on the measurement of isotope ratios in samples where the isotopic composition has been altered by the addition of a known amount of an isotopically enriched element [1]. It is an absolute
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Comparative study on quantitation of human myoglobin by both isotope
Isotope dilution mass spectrometry (IDMS) is a classical primary method for protein quantitation based on the primary structure of the target protein [7,8,9,10,11]. For IDMS, pure peptides or proteins are usually hydrolyzed into amino acid mixtures and the concentration of stable amino acids is quantified by adding isotopic amino acids as ...
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Isotope dilution mass spectrometry for absolute quantification in
Isotope dilution mass spectrometry is a reference technique for quantitative analysis, given that it combines the sensitivity and selectivity of MS instruments with the precision and accuracy associated with the use of internal standards. Isotope-labeled proteins are the optimal internal standards for quantitative proteomics as they …
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An isotope dilution mass spectrometry overview: tips and …
The mass fraction and mass fraction ratio are then converted into activity ratios by considering the radionuclide atomic masses and their respective half-lives (t 1/2).It should be noted that the double isotope dilution in combination with a double spike (i.e. 229 Th– 236 U) is a powerful tool that helps to minimize weighing errors and eliminate errors due …
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Principles and Applications of Liquid Chromatography-Mass Spectrometry
Liquid chromatography-mass spectrometry (LC-MS) is now a routine technique with the development of electrospray ionisation (ESI) providing a simple and robust interface. ... McCoy L, Zhang M, Gunter EW. Determination of folate vitamers in human serum by stable-isotope-dilution tandem mass spectrometry and comparison with radioassay and ...
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